NativeSkin® Human Skin Models

LIVE HUMAN SKIN ADAPTED TO YOUR NEEDS

REAL HUMAN SKIN STRUCTURE

NativeSkin® human skin models hold normal skin barrier function, a mature stratum corneum, a functional basal layer and all cell types and skin appendages of in vivo human skin.

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LIVE HUMAN SKIN RESPONSE

After 7 days of culture, NativeSkin models still reflect the same histological features as in vivo human skin to allow for repeated testing.

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READY. EASY. STANDARDIZED.

NativeSkin is designed as a ready-to-use testing kit for easy manipulation. Production is standardized for reproducible results.

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ADAPTED TO YOUR NEEDS

NativeSkin can be used for both systemic and topical applications, without leaking into the medium.

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NativeSkin

The last-line screens prior to clinical evaluation

NativeSkin is a patented human skin model to simplify the culture, treatment and manipulation of human skin for research and analysis. NativeSkin kits contain round human skin biopsies from surgical residue donated by adult volunteers. Every individual skin biopsy is embedded in a high-tech solid matrix that maintains and nourishes the skin during shipment. A chemically defined animal component-free (ACF) / xeno-free (XF) skin culture medium is also provided in the kit that needs to be changed every day to keep the skin biopsies alive for 7 days. NativeSkin helps you avoid animal experimentation and represents a highly predictive and cost-effective last-line screen in laboratory conditions prior to in vivo clinical evaluations.

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Nativeskin models are the answer to assays challenges with living human skin

Applications

The culture of skin explants has a long tradition in investigative dermatology to study the physiology and pathophysiology of human skin and epidermal appendages in vitro. NativeSkin is relevant for the following applications:

Efficacy testing
Toxicology testing
Topical application
Dermal studies
Epidermal studies

Percutaneous absorption
Metabolism
Repeated dose assay
Long term protocols
Langerhans cells study

Skin resident T cells study
Immune responses
Melanogenesis
Melanocyte study

Approved protocols

Genoskin has approved different protocols to analyze the effects of a treatment or compound on NativeSkin models:

Viability assay (MTT/LDH)
Formalin fixation for paraffin embedding
Epidermal separation with Dispase
Transdermal permeation assays

Immunofluorescence
In-situ immunofluorescence
Cytokines & chemokines release (ELISA/MSD)

RNA extraction
Protein extraction

FOR MORE DETAILED PROTOCOLS…

Please don’t hesitate to contact us.

NativeSkin is available in 4 different sizes

ex-vivo human skin model human Nativeskin come in 4 different sizes to provide better human data

NativeSkin comes in different sizes and versions to answer all your study and assay requirements.

The standard version of NativeSkin models is designed for skin absorption studies and contains a white silicone ring that is firmly fixed to the skin biopsy. This silicone ring covers the outer edge of the biopsy to prevent lateral diffusion of topically applied products. A more basic version and a smaller model (NativeSkin S), without the silicone ring, are also available and designed for systemic treatments and genomic readout. Below you will find an overview of our entire range of NativeSkin products.

REFERENCE	DESCRIPTION	WORKING SURFACE	DIAMETER	RECOMMENDED TOPICAL VOLUME	EX VIVO CULTURE DURATION	PLATE FORMAT	REQUIRED VOLUME	MINIMUM ORDER
NSA08	NativeSkin Access (w/o silicone ring)	–	8 mm	–	7 days	12 wells	1mL	n.a.
NSA11	NativeSkin Access	0.5 cm2	11 mm	10-20uL	7 days	12 wells	1mL	n.a.
NS004	NativeSkin Medium	1.13 cm2	15 mm	25-45uL	7 days	6 wells	2mL	12 wells
NS005	NativeSkin Large	1.76 cm2	20 mm	50-70uL	7 days	6 wells	2mL	12 wells
NS006	NativeSkin XL	2.54 cm2	23 mm	100-200uL	5 days	6 wells	3mL	12 wells