NativeSkin® Human Skin Models
LIVE HUMAN SKIN ADAPTED TO YOUR NEEDS
REAL HUMAN SKIN STRUCTURE
NativeSkin® human skin models hold normal skin barrier function, a mature stratum corneum, a functional basal layer and all cell types and skin appendages of in vivo human skin.
LIVE HUMAN SKIN RESPONSE
After 7 days of culture, NativeSkin models still reflect the same histological features as in vivo human skin to allow for repeated testing.
READY. EASY. STANDARDIZED.
NativeSkin is designed as a ready-to-use testing kit for easy manipulation. Production is standardized for reproducible results.
ADAPTED TO YOUR NEEDS
NativeSkin can be used for both systemic and topical applications, without leaking into the medium.
Applications
The culture of skin explants has a long tradition in investigative dermatology to study the physiology and pathophysiology of human skin and epidermal appendages in vitro. NativeSkin is relevant for the following applications:
- Efficacy testing
- Toxicology testing
- Topical application
- Dermal studies
- Epidermal studies
- Percutaneous absorption
- Metabolism
- Repeated dose assay
- Long term protocols
- Langerhans cells study
- Skin resident T cells study
- Immune responses
- Melanogenesis
- Melanocyte study
Approved protocols
Genoskin has approved different protocols to analyze the effects of a treatment or compound on NativeSkin models:
- Viability assay (MTT/LDH)
- Formalin fixation for paraffin embedding
- Epidermal separation with Dispase
- Transdermal permeation assays
- Immunofluorescence
- In-situ immunofluorescence
- Cytokines & chemokines release (ELISA/MSD)
- RNA extraction
- Protein extraction
NativeSkin is available in 4 different sizes
NativeSkin comes in different sizes and versions to answer all your study and assay requirements.
The standard version of NativeSkin models is designed for skin absorption studies and contains a white silicone ring that is firmly fixed to the skin biopsy. This silicone ring covers the outer edge of the biopsy to prevent lateral diffusion of topically applied products. A more basic version and a smaller model (NativeSkin S), without the silicone ring, are also available and designed for systemic treatments and genomic readout. Below you will find an overview of our entire range of NativeSkin products.
| REFERENCE | DESCRIPTION | WORKING SURFACE | DIAMETER | RECOMMENDED TOPICAL VOLUME | EX VIVO CULTURE DURATION | PLATE FORMAT | REQUIRED VOLUME | MINIMUM ORDER |
|---|---|---|---|---|---|---|---|---|
| NSA08 | NativeSkin Access (w/o silicone ring) | – | 8 mm | – | 7 days | 12 wells | 1mL | n.a. |
| NSA11 | NativeSkin Access | 0.5 cm2 | 11 mm | 10-20uL | 7 days | 12 wells | 1mL | n.a. |
| NS004 | NativeSkin Medium | 1.13 cm2 | 15 mm | 25-45uL | 7 days | 6 wells | 2mL | 12 wells |
| NS005 | NativeSkin Large | 1.76 cm2 | 20 mm | 50-70uL | 7 days | 6 wells | 2mL | 12 wells |
| NS006 | NativeSkin XL | 2.54 cm2 | 23 mm | 100-200uL | 5 days | 6 wells | 3mL | 12 wells |
